Florovsky Monastery was first mentioned in the documents of the XVI century, at least in 1566 it was granted charter Duke Konstantin Ostrog, according to which the monastery was transferred to Jacob archpriest Gulkevichi, which resumed activities of the monastery (that is, it existed before). In 1682 already it is mentioned that at the hem was a nunnery with the two churches, one of which bore the name of the martyr Flora.

However, since the resumption of the activities of the monastery prior to the XVIII century at the Florovsky monastery it was difficult to finance, so it is hardly developed. Only in 1712, after the closure of the women's Resurrection Monastery and the transfer of the nuns who lived there in the Florovsky Monastery begins flowering of the monastery, as all the private ownership of the monastery became the property of Florovsky.

Shortly after the transfer of the nuns at the Florovsky monastery begin to build a new one, now the stone Church of the Ascension. After the consecration of the church in 1732, the monastery began officially called the Holy Ascension Florovsky. Besides everything else the temple was made of wood, so burned in the famous Kiev fire in 1811. The following year, the treasury has been allocated funds for the restoration of the monastery, which were erected stone buildings. By the beginning of the twentieth century have the entire territory of the monastery was built of stone and wooden buildings (hospital, an almshouse and a few temples).

During the Soviet period the monastery was closed, destroying the temple of the Holy Trinity. The revival of the monastery began only during the German occupation, and although later Kiev was liberated, the monastery is not closed, though, and continued to suffer harassment from the authorities. Today, it continues to evolve and develop the traditions of spirituality.